大菱鲆2种类胰岛素样生长因子结合蛋白基因克隆及在成鱼和早期发育期中的表达     

胡健  温海深  关健  官曙光  何峰  李吉方  史丹  马瑞芹  刘淼  母伟杰  张远青 《海洋学报》2012,34(5):139-146

类胰岛素样生长因子结合蛋白(IGFBP)在调节IGF的生物学功能上起着重要的作用,利用简并引物扩增及RACE克隆技术,首次克隆得到大菱鲆IGFBP-1,-2基因cDNA全长序列。两个基因氨基酸序列都分为保守的N端和C端以及高度特异的L区域3个部分,其中N端和C端共包含18个半胱氨酸残基,N端存在一段保守的(GCGCCXXC)结构,C端存在一段保守的(CWCV)结构,这些结构在与IGF相互作用以及调节与IGF结合的亲和性和稳定性起重要作用。组织表达分析表明,igfbp-1主要在肝脏及脾中大量表达,在其他组织中表达量较少,igfbp-2除在肌肉中没有检测到表达外,在其他组织中均有表达,其中肝脏脑、心、肾、肠中表达量较高。广泛的组织表达模式表明IGFBP是通过自分泌和旁分泌来调节IGF的功能。对于胚胎发育期表达分析表明,igfbp-1在整个发育期均检测到表达,igfbp-2在胚体期开始有表达,结果表明igfbp-1和igfbp-2在胚胎发育过程中的细胞生长以及组织器官分化都起着重要的生理学作用。  相似文献   

Genetic difference of Chinese horseshoe crab (Tachypleus tridentatus) in southeast coast of China based on mitochondrial COI gene analysis     

WENG Zhaohong  XIAO Zhiqun  XIE Yangjie  WANG Zhiyong  GUI Jianfang 《海洋学报(英文版)》2012,31(3):132-137

Population genetic structure and historical demography of Chinese horseshoe crab (T. tridentatus) along southeast coast of China were inferred from the sequence data of mitochondrial cytochrome c oxidase subunit I (COI) fragment. The sequence analysis for 964 bp COI fragment was conducted in 28 individuals collected from five localities: Ninghai in Zhejiang Province, Meizhou and Zhangpu in Fujian Province, Beihai of Guangxi Zhuang Autonomous Region and Danzhou of Hainan Province. Sequence variation was relatively low with a total of seven transitions observed. In all localities, Haplotype H3 was the dominant type observed among eight haplotypes defined previously, and was at the center of radiation in Median-Joining network. The prolonged star-like network suggests a signature of population expansions. The level of diversity was low in total, with haplotype diversity (H_d) being equal to 0.765 and nucleotide diversity (π) being equal to 0.001 18, respectively. The genetic structure analysis revealed the significant genetic difference between Ninghai and Danzhou populations. Both mismatch distribution analysis and Fu's F_s test provided consistent inference of historic population expansion. The low genetic diversity of horseshoe crab observed along China coast indicated that urgent measures should be taken to protect this rare marine animal.  相似文献   

Morphological observation and rbcL gene sequences studies of two new species, Grateloupia dalianensis H.W.Wang et D.Zhao, sp. nov. and G. yinggehaiensis H.W.Wang et R.X.Luan, sp. nov. (Halymeniaceae, Rhodophyta) from China     

ZHAO Dan  WANG Hongwei  SHENG Yingwen  L&#; Jianzhou  LUAN Rixiao 《海洋学报(英文版)》2012,31(2):109-120

A few species in the genus Grateloupia (Halymeniaceae,Rhodophyta) have been investigated in detail with respect to morphological observations and molecular analyses.In this study,the authors document the vegetative and reproductive structures of two new species of Grateloupia,G.dalianensis H.W.Wang et D.Zhao,sp.nov.and G.yinggehaiensis H.W.Wang et R.X.Luan,sp.nov.They both have the morphological character that carpogonial ampullae and auxiliary cell ampullae are the simple Grateloupia-type.The two species can be distinguished from other species of the genus by their distinctive morphological features respectively.Based on ribulose-1,5-bisphosphate carboxylase/oxygenase (rbcL) gene sequences,the phylogenetic tree obtained in the study indicated that they are both embedded within the Grateloupia clade.G.dalianensis clusters a subclade with G.asiatica,and G.yinggehaiensis forms a single monophyletic subclade with G.hawaiiana.  相似文献   

刺参虾青素基因的克隆及不同体色个体间表达差异的分析     

赵鹤凌  杨红生  赵欢  刘石林 《海洋科学》2012,36(3):22-28

仿刺参(Apostichopus japonicus Selenka)的体色变化很大,多数背腹均为黄褐色,极少数呈白化特征,背腹均为纯白色。经人工繁育实验发现,白化仿刺参的子代仍多具白化特征。本文研究了虾青素基因与刺参白化特征的相关性。在克隆虾青素基因cDNA全长的基础上,比较了普通仿刺参和白化仿刺参在不同发育时期虾青素基因表达量的差异。结果表明,该基因的cDNA含有2058个核苷酸,编码560个氨基酸。经实时定量PCR分析,白化成参体壁中虾青素基因表达量显著低于普通成参;而在仿刺参色素沉积的早期,白化稚参体壁中虾青素基因表达量从受精后第39天开始显著低于普通稚参。可见,仿刺参体壁中虾青素基因的低表达与刺参白化特征的发生密切相关。  相似文献   

Mutations in Exons of the CYP17-Ⅱ Gene Affect Sex Steroid Concentration in Male Japanese Flounder（Paralichthys olivaceus）     

MA Ruiqin  HE Feng  WEN Haishen  LI Jifang  SHI Bao  SHI Dan  LIU Miao  MU Weijie  ZHANG Yuanqing  HU Jian  HAN Weiguo  ZHANG Jianan  WANG Qingqing  YUAN Yuren  LIU Qun 《青岛海洋大学学报(英文版)》2012,(1):99-104

  相似文献   

TRACK��ƵGPS��λ����ʱ�ο�վ��ѡȡ����   总被引：3，自引：0，他引：3  

殷海涛  肖根如  张磊  朱成林 《大地测量与地球动力学》2012,32(4):15-19

??????TRACK????????????λ????????????ο???????????GPS????????е?????????????????????????????????????????????????·??Ч?С??0.2 m???????????????????????????????????????ο??????????????λ?????????????????????GIPSY??PANDA????????????????????  相似文献   

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钟斌  吴云 《大地测量与地球动力学》2012,32(4):57-60

????????GPS??????????????????IGU??????????????÷???????????????????????????????????????????????λ?????????????????????????????????IGS??????????????侫????????0.2 ns??????λ???????????????1 cm??????????????2 cm??  相似文献   

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程广义  张海东  张英利  万鑫  赵淑芬 《大地测量与地球动力学》2012,32(2):96-99

???????GPS???????????????泤??????????????????????????????GPS????????????????????GPS???????????辫??????????????????GPS??????衣???????????GPS????????????????????????????????????????????  相似文献   

阿克苏河流域气候变化对潜在蒸散量影响评价(英文)     

张守红  刘苏峡  莫兴国  舒畅  孙杨  张春 《地理学报(英文版)》2011,21(4):609-620

Evapotranspiration is one of the key components of hydrological processes. Assessing the impact of climate factors on evapotranspiration is helpful in understanding the impact of climate change on hydrological processes. In this paper, based on the daily meteorological data from 1960 to 2007 within and around the Aksu River Basin, reference evapotranspiration (RET) was estimated with the FAO Penman-Monteith method. The temporal and spatial variations of RET were analyzed by using ARCGIS and Mann-Kendall met...  相似文献   

阿克苏河流域气候变化对潜在蒸散量影响分析   总被引：13，自引：1，他引：12  

张守红  刘苏峡  莫兴国  舒畅  孙杨  张春 《地理学报》2010,65(11):1363-1370

蒸散发是水文过程的关键环节,研究气候因子对潜在蒸散发的影响,有助于深入认识水文过程对气候变化的响应。本文基于阿克苏河流域1960-2007 年逐日气象资料和Penman-Monteith公式,估算并分析参考作物蒸散量(RET) 时空变化特征,并用多元回归方法定量区分气候因子变化对RET 变化的贡献率。研究发现流域RET 空间差异明显,东部平原区平均年RET 为1100mm左右,是西部山区的近2 倍;东南部绿洲区的RET显著减少,而西部变化复杂。RET变化趋势的季节差异也很显著,以夏季变幅最大,是年变化的主要贡献者。高海拔地区相对湿度对RET变化影响最大,其它区域的风速变化对RET变化的贡献率最高。库车和乌恰站的风速变化对RET变化的贡献率大于50%,是RET变化的主导因素。  相似文献